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Principle >>
With wavelengths of 405, 470, and 580nm, the 405nm is used as a reference channel to control the data to effectively remove motion noise, while the 470 and 580nm can excite two types of fluorescent proteins (e.g., calcium-sensitive fluorescent proteins or neurotransmitter probes, etc.), which can be used to detect the changes of different ions (e.g., calcium ions) or neurotransmitter concentrations (e.g., dopamine, epinephrine, etc.), respectively.The system can be used in in vivo animals to detect the level of signaling changes associated with different neuronal cell populations, to reflect the coding characteristics of different types of neurons in the same behavioral paradigm, and to validate connections between neurons or correlations between signaling pathways.
