Principle >>
It is mainly used for in vivo calcium imaging of animals to study the relationship between neuronal circuits in different parts of the brain and behavior. It intuitively reflects the activation state of corresponding brain regions under various behavioral paradigms, and provides real-time observation of blood vessels and extracellular mesenchyme in deep brain regions. It can accomplish the following functions: real-time observation of neural projection activities during complex behaviors; calcium imaging in deep brain regions; calcium imaging in the cortex; and changes in migration of fluorescent cells in deep brain regions.

System function features and advantages >>
◆ System components include microscope body, fixation plate, GRIN lens, CMOS, image acquisition card and acquisition software, steering gear, etc;
◆ Recording calcium signals from a group of neurons at the single-cell resolution level;
◆  Suitable for in vivo experiments on free-moving animals;
◆  Deep brain imaging can be realized by implanting GRIN lens;
◆  The system is small in size and light in weight, and does not affect the free movement of mice and behavioral experiments.
Workflow >>
◆  GCaMP6 or other calcium fluorescence indicator by virus injection, GRIN lens was implanted and waiting for virus expression.
◆ The activity of nerve cells leads to the increase of intracellular calcium ion concentration, which increases the fluorescence intensity of fluorescent probes, such as GCaMP6. The fluorescence is collected through the implanted lens, converted into image signals by the CMOS, and captured by a high-speed image acquisition card.
◆ Image processing software further analyzed the correlation of neural cell activity and behavior.